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Oxford Instruments imaris 3d reconstruction images

MES enhances peripheral nerve regeneration by recruiting NCSCs-like cells at the injury site (A–C) Representative <t>3D</t> reconstructed PS-OCT images of sciatic nerves (A) without or (B) with MES in a rat sciatic transection model. The nerve conduit is indicated by translucent gray while the nerve is indicated by red-brown color. A normal sciatic nerve wrapped in the conduit was used as a (C) HC. After conduit transplantation onto the transected sciatic nerve, the injury site was subjected to MES using therapeutic shockwave (1,000 pulses per time, twice a week) for 12 weeks. The PS-OCT images and immunohistochemistry staining were conducted 12 weeks post-surgery. (D) Quantification of phase retardation of PS-OCT images at proximal and distal ends ( n = 3, mean <t>±</t> <t>SEM).</t> (E–G) Representative immunohistochemical images showing the expression of neural crest markers SOX2, P75-NTR, and nerve regeneration factor NRG1 at the proximal/distal ends and the middle of the conduits bridging transected sciatic nerves under (E) static, (F) MES, or (G) HC conditions. (H) Representative zoomed in images of nuclear SOX2. (I–K) Quantification of histology staining intensities of (I) nuclear SOX2, (J) P75, and (K) NRG1. ∗ and ∗∗ denote statistical significance of p < 0.05 and p < 0.01. After conduit transplantation onto the transected sciatic nerve, the injury site was subjected to MES using therapeutic shockwave (1,000 pulses per time, twice a week) for 12 weeks. The PS-OCT images and immunohistochemistry staining were conducted 12 weeks post-surgery. Quantification data were collected from a total of 9 images from 3 rats for each condition.

Imaris 3d Reconstruction Images, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3d+image+reconstruction/Imaris/pmc13083801-55-41-41
Average 99 stars, based on 44266 article reviews

imaris 3d reconstruction images - by Bioz Stars, 2026-09

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Images

1) Product Images from "The regenerative role of neural crest stem cells in physical stimuli-enhanced peripheral nerve repair"

Article Title: The regenerative role of neural crest stem cells in physical stimuli-enhanced peripheral nerve repair

Journal: Stem Cell Reports

doi: 10.1016/j.stemcr.2026.102861

MES enhances peripheral nerve regeneration by recruiting NCSCs-like cells at the injury site (A–C) Representative 3D reconstructed PS-OCT images of sciatic nerves (A) without or (B) with MES in a rat sciatic transection model. The nerve conduit is indicated by translucent gray while the nerve is indicated by red-brown color. A normal sciatic nerve wrapped in the conduit was used as a (C) HC. After conduit transplantation onto the transected sciatic nerve, the injury site was subjected to MES using therapeutic shockwave (1,000 pulses per time, twice a week) for 12 weeks. The PS-OCT images and immunohistochemistry staining were conducted 12 weeks post-surgery. (D) Quantification of phase retardation of PS-OCT images at proximal and distal ends ( n = 3, mean ± SEM). (E–G) Representative immunohistochemical images showing the expression of neural crest markers SOX2, P75-NTR, and nerve regeneration factor NRG1 at the proximal/distal ends and the middle of the conduits bridging transected sciatic nerves under (E) static, (F) MES, or (G) HC conditions. (H) Representative zoomed in images of nuclear SOX2. (I–K) Quantification of histology staining intensities of (I) nuclear SOX2, (J) P75, and (K) NRG1. ∗ and ∗∗ denote statistical significance of p < 0.05 and p < 0.01. After conduit transplantation onto the transected sciatic nerve, the injury site was subjected to MES using therapeutic shockwave (1,000 pulses per time, twice a week) for 12 weeks. The PS-OCT images and immunohistochemistry staining were conducted 12 weeks post-surgery. Quantification data were collected from a total of 9 images from 3 rats for each condition.

Figure Legend Snippet: MES enhances peripheral nerve regeneration by recruiting NCSCs-like cells at the injury site (A–C) Representative 3D reconstructed PS-OCT images of sciatic nerves (A) without or (B) with MES in a rat sciatic transection model. The nerve conduit is indicated by translucent gray while the nerve is indicated by red-brown color. A normal sciatic nerve wrapped in the conduit was used as a (C) HC. After conduit transplantation onto the transected sciatic nerve, the injury site was subjected to MES using therapeutic shockwave (1,000 pulses per time, twice a week) for 12 weeks. The PS-OCT images and immunohistochemistry staining were conducted 12 weeks post-surgery. (D) Quantification of phase retardation of PS-OCT images at proximal and distal ends ( n = 3, mean ± SEM). (E–G) Representative immunohistochemical images showing the expression of neural crest markers SOX2, P75-NTR, and nerve regeneration factor NRG1 at the proximal/distal ends and the middle of the conduits bridging transected sciatic nerves under (E) static, (F) MES, or (G) HC conditions. (H) Representative zoomed in images of nuclear SOX2. (I–K) Quantification of histology staining intensities of (I) nuclear SOX2, (J) P75, and (K) NRG1. ∗ and ∗∗ denote statistical significance of p < 0.05 and p < 0.01. After conduit transplantation onto the transected sciatic nerve, the injury site was subjected to MES using therapeutic shockwave (1,000 pulses per time, twice a week) for 12 weeks. The PS-OCT images and immunohistochemistry staining were conducted 12 weeks post-surgery. Quantification data were collected from a total of 9 images from 3 rats for each condition.

Techniques Used: Transplantation Assay, Immunohistochemistry, Staining, Immunohistochemical staining, Expressing

MES induces the multiphenotypic differentiation of NCSCs-like cells toward neurons and Schwann cells in vitro (A) Gene expression of neuronal markers NEUROD1 , MASH1 , NGN2 , and Schwann cell markers KROX20 , NCAM1 , PMP22 after 1 week of culture under the control (C), biochemical factor (BC), MES, and MES+BC conditions. n = 4 (biologically independent), mean ± SEM. (B) Confocal images showing the expression of neuronal markers (beta III tubulin [TUJ. 1] and NEUN) after 0, 1, and 2 weeks of culture under the control, BC, MES, and MES+BC conditions. (C) Confocal images showing the expression of a neuronal marker TUJ. 1, a Schwann cell marker GALC after 2 weeks of culture under the control, BC, MES, and MES+BC conditions. (D) Quantification of axon length and GALC fluorescence intensity. n = 3 (biologically independent), mean ± SEM. (E) Confocal images and corresponding Imaris 3D reconstruction images of the cells after 4 weeks of culture under the control, BC, MES, and MES+BC conditions, the cells were fluorescently labeled by TUJ. 1 and GALC. NCSC-like cells were subjected to acoustic actuator stimulation as MES, biochemical factor NRG1 stimulation as BC, or the combination of both as MES+BC. The cells were stimulated for 2 h daily for either 2 weeks or 4 weeks ∗ and ∗∗ denote statistical significance of p < 0.05 and p < 0.01, respectively. ∗ and ∗∗ denote statistical significance p < 0.05 and p < 0.01, respectively.

Figure Legend Snippet: MES induces the multiphenotypic differentiation of NCSCs-like cells toward neurons and Schwann cells in vitro (A) Gene expression of neuronal markers NEUROD1 , MASH1 , NGN2 , and Schwann cell markers KROX20 , NCAM1 , PMP22 after 1 week of culture under the control (C), biochemical factor (BC), MES, and MES+BC conditions. n = 4 (biologically independent), mean ± SEM. (B) Confocal images showing the expression of neuronal markers (beta III tubulin [TUJ. 1] and NEUN) after 0, 1, and 2 weeks of culture under the control, BC, MES, and MES+BC conditions. (C) Confocal images showing the expression of a neuronal marker TUJ. 1, a Schwann cell marker GALC after 2 weeks of culture under the control, BC, MES, and MES+BC conditions. (D) Quantification of axon length and GALC fluorescence intensity. n = 3 (biologically independent), mean ± SEM. (E) Confocal images and corresponding Imaris 3D reconstruction images of the cells after 4 weeks of culture under the control, BC, MES, and MES+BC conditions, the cells were fluorescently labeled by TUJ. 1 and GALC. NCSC-like cells were subjected to acoustic actuator stimulation as MES, biochemical factor NRG1 stimulation as BC, or the combination of both as MES+BC. The cells were stimulated for 2 h daily for either 2 weeks or 4 weeks ∗ and ∗∗ denote statistical significance of p < 0.05 and p < 0.01, respectively. ∗ and ∗∗ denote statistical significance p < 0.05 and p < 0.01, respectively.

Techniques Used: In Vitro, Gene Expression, Control, Expressing, Marker, Fluorescence, Labeling

Software:

Article Title: CD9 regulates macrophage-mediated remodeling of adipose tissue in obesity.
Article Snippet: .. 3D image reconstruction was performed in Imaris 549 software (v8.1.2, Oxford Instruments). ..

Article Title: Comprehensive analysis of keloid vasculature by tissue clearing and 3D imaging
Article Snippet: .. The captured Z‐stack images were processed using ImageJ software version 1.5.4 (National Institutes of Health, Bethesda, MD, USA), and 3D image reconstruction was performed using Imaris version 10.0.1 software (Oxford Instruments, Abingdon, UK) with a voxel size of 3.45 × 3.45 × 10 μm 3 . ..

Generated:

Article Title: EPHA2 and scavenger receptor-directed trafficking enhances endosomal leakiness and antisense therapy delivery
Article Snippet: .. For 3D image reconstruction, Imaris software was used to create the 3D surfaces using the SIM data generated after processing the images obtained using the Elyra7 microscope. .. H1299 cells were transfected with scavenger receptor siRNA pools using nucleofection Kit V and an AMAXA nucleofector (Lonza).

Microscopy:

Article Title: Peptide compound with repetitive sequences
Article Snippet: .. Z-stack images are acquired using an inverted laser scanning confocal microscope (ZeissTM LSM 880 Inverted Confocal Microscope, Germany), and 3D image reconstruction is performed using Imaris software. ..

Imaging:

Article Title: Topological segregation of stress sensors along the gut crypt-villus axis.
Article Snippet: The crypt–villus structure of the small intestine serves as an essential protective barrier.. The integrity of this barrier is monitored by the complex sensory system of the gut, in which serotonergic enterochromaffin (EC) cells play an important part.. These rare sensory epithelial cells surveil the mucosal environment for luminal stimuli and transmit signals both within and outside the gut.

Cloning:

Article Title: Phase separation of initiation hubs on cargo is a trigger switch for selective autophagy.
Article Snippet: .. We thank A. Simonsen and S. Migliano from the Stenmark laboratory for providing plasmids, B. Lace from the Ott laboratory for help with 3D image reconstruction using Nature Cell Biology Article https://doi.org/10.1038/s41556-024-01572-y the Imaris software, I. Valiya-Parambath for help with cloning and M. McDowell for advice on mutating the GBP–GFP interaction surface. ..

Staining:

Article Title: CD9 regulates macrophage-mediated remodeling of adipose tissue in obesity
Article Snippet: .. All fluorescent staining (blue, green, red, and far-red channels) were acquired using HyD detectors in the standard mode with 100% gain, and 3D image reconstruction was performed with Imaris software (v8.1.2, Oxford Instruments). ..

other:

Article Title: Development of Novel Technology for the Visualization and Quantitation of Angiogenesis and the Alveolar-Capillary Network in a Mouse Model of Fibrosis.
Article Snippet: Affiliations: 1Department of Medicine, Division of Pulmonary, Critical Care & Sleep Medicine, National Jewish Health, Denver CO, 80206; 2Cell, Stem Cell and Development Graduate Program, University of Colorado Anschutz Medical Campus, Aurora, CO; 3Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO; 4Onimagin Technologies SCA, Cordoba, Spain; 5Gates Center for Regenerative Medicine and Stem Cell Biology, University of Colorado, Aurora, CO, 80045.

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